Genome Sequencer FLX (Roche/454)

Sequencing applications

• Re-sequencing or de-novo sequencing of entire genomes
• Transcriptome sequencing
• Sequencing of genomic target regions. Please contact us to discuss enrichment
• Multiplex sequencing

Our sequencing service includes

• Preparation of libraries from gDNA or RNA for shotgun sequencing.
• Emulsion PCR
• Sequence runs using the Titanium 70 chemistry.
• Delivery of sequence reads with quality information using the UPPNEX project at SNIC-UPPMAX.
• Bioinformatics support includes data filtering, basic alignment

Starting material

Depending on the application the sample is DNA, RNA, or enriched DNA or PCR-products. The amount and quality of the samples is pivotal to the outcome of sequencing. The amount and quality of each sample will be verified by the SNP&SEQ platform prior to library preparation, or prior to sequencing if a library is submitted to the platform.

Amount of data generated

With the current version of instrument, software and reagents, sequencing using the Genome Sequencer FLX generates approximately 500 Mbases of sequence data per run. The reads are on average/median 400bases long (range 100-800 bases)

Delivery of data

Second generation sequence data is handled by dedicated servers equipped with large capacity redundant storage. For delivery of the large amounts of data generated by second generation sequencing we collaborate with SNIC-UPPMAX, Uppsala University's resource of high-performance computers, in the UPPNEX project. Quality controlled data will be made available via a project account created for each user at UPPNEX. The user can then perform further data analysis using the SNIC-UPPMAX compute clusters. Upon request, data can also be delivered on USB-disks or via an FTP-server.

Pricing

The cost for a sequencing project consists of the cost for library preparation including titration and emulsion PCR and that of sequencing. The cost varies with the application and the number of samples to be analysed. Please contact Ulrika Liljedahl for the specific pricing for your project.

Contact

To get access to our services, please complete a project description form and send it to our sequencing manager. For more information on our service or additional information on the different sequencing applications, please contact our sequencing manager.